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Total branch length and spine density were determined using Volocity software Improvision in 3D restored confocal z-stacks 1024 artikkel kilde 1024 resolution that went through deconvolution to remove noise pixels. Case demographics of PTSD patients and matched healthy control subjects. Gene expression profiling in postmortem prefrontal cortex of major depressive disorder. Neurocircuitry models of posttraumatic stress disorder and extinction: Neural correlates of exposure to traumatic pictures and sound in Vietnam combat veterans with and without posttraumatic stress disorder: Linken addition, morphological studies conducted in rodent mPFC show that chronic stress causes a reduction of dendrite length and spine density of pyramidal neurons, and these alterations are associated with impairments in mPFC-mediated cognitive tasks [ 16 ].

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As SGK1 expression is induced by glucocorticoids, we measured protein levels of glucocorticoid receptor GR in the PFC and found no significant changes between naïve, high- and low-escape rats S2 Fig. The results also demonstrate that inhibition of SGK1 in the mPFC causes behavioral deficits characteristic of traumatic stress. Statistical expression analysis of microarray image files was performed using GeneSpring GX 7. Inescapable shock, neurotransmitters, and addiction to trauma: After 3 x 15 min washes, the slices were incubated for 2 h with secondary Alexa Fluor 488 and 546-conjugated antibodies Jacson ImmunoResearch and Invitrogen, respectivelyand confocal images were collected.

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Journal of Experimental Psychology: On days 1 and 2, the rats were habituated to the conditioning chambers for 10-min. Inescapable stress, originally recognized as an animal model for depression, produces a syndrome that shares many behavioral, physiologic, and neurochemical features observed in MDD and PTSD [ 12 — 15 ]. Each PFC hemisphere was infused with 3 μl of titer adjusted viral stocks at a rate of 0. The whole genome expression analysis was performed using two-channel MI Ready microarrays Microarray, Huntsville, Alabama. F There was no difference in the locomotor activity between the groups LA.

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